TMB ELISA Substrate – HE101
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TMB ELISA Substrate
TMB ELISA Substrate is a ready-to-use chromogenic substrate for detection of horseradish peroxidase (HRP) activity. HRP can catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) to yield a blue color, the maximal absorbance is at 370 nm or 620-652 nm; however, upon addition of the stop solution, the solution turns to yellow and can be measured at 450 nm.
Manual and datasheet