Human VEGF-A (Vascular Endothelial Cell Growth Factor A ) ELISA Kit – E-EL-H0111

Human VEGF-A (Vascular Endothelial Cell Growth Factor A ) ELISA Kit

The Human VEGF-A ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-A . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and then incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-A , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGF-A . You can calculate the concentration of Human VEGF-A in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Vascular Endothelial Cell Growth Factor A

Abbreviation : VEGF-A

Uniprot ID : P15692

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

More information about this Human VEGF-A (Vascular Endothelial Cell Growth Factor A ) ELISA Kit from Elabscience