Human UGCG (UDP-glucose ceramide glucosyltransferase) ELISA Kit – E-EL-H1778

Human UGCG (UDP-glucose ceramide glucosyltransferase) ELISA Kit

The Human UGCG ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human UGCG. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human UGCG and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UGCG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human UGCG. You can calculate the concentration of Human UGCG in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: UDP-glucose ceramide glucosyltransferase

Abbreviation : UGCG

Uniprot ID : Q16739

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.938 ng/ml

Detection Range : 1.563 – 100 ng/ml

Manual & Datasheet

More information about this Human UGCG (UDP-glucose ceramide glucosyltransferase) ELISA Kit from Elabscience