Human UCRP (Ubiquitin Cross Reactive Protein) ELISA Kit – E-EL-H2531

Human UCRP (Ubiquitin Cross Reactive Protein) ELISA Kit

The Human UCRP ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to UCRP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for UCRP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UCRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of UCRP. You can calculate the concentration of UCRP in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Ubiquitin Cross Reactive Protein

Abbreviation : UCRP

Uniprot ID : P05161

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

More information about this Human UCRP (Ubiquitin Cross Reactive Protein) ELISA Kit from Elabscience