Human UBE2C (Ubiquitin Conjugating Enzyme E2C) ELISA Kit – E-EL-H1256

Human UBE2C (Ubiquitin Conjugating Enzyme E2C) ELISA Kit

The Human UBE2C ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to UBE2C. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for UBE2C and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UBE2C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of UBE2C. You can calculate the concentration of UBE2C in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Ubiquitin Conjugating Enzyme E2C

Abbreviation : UBE2C

Uniprot ID : O00762

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Human UBE2C (Ubiquitin Conjugating Enzyme E2C) ELISA Kit from Elabscience