Human UBE2A (Ubiquitin Conjugating Enzyme E2A) ELISA Kit – E-EL-H2381

Human UBE2A (Ubiquitin Conjugating Enzyme E2A) ELISA Kit

The Human UBE2A ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to UBE2A. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for UBE2A and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UBE2A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of UBE2A. You can calculate the concentration of UBE2A in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Ubiquitin Conjugating Enzyme E2A

Abbreviation : UBE2A

Uniprot ID : P49459

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

More information about this Human UBE2A (Ubiquitin Conjugating Enzyme E2A) ELISA Kit from Elabscience