Human TSP-1 (Thrombospondin-1) ELISA Kit – E-EL-H1589

Human TSP-1 (Thrombospondin-1) ELISA Kit

The Human TSP-1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TSP-1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TSP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TSP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TSP-1. You can calculate the concentration of TSP-1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Thrombospondin-1

Abbreviation : TSP-1

Uniprot ID : P07996

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 4.688 ng/ml

Detection Range : 7.813 – 500 ng/ml

Manual & Datasheet

More information about this Human TSP-1 (Thrombospondin-1) ELISA Kit from Elabscience