Human TRH (Thyrotropin Releasing Hormone) ELISA Kit – E-EL-H1142

Human TRH (Thyrotropin Releasing Hormone) ELISA Kit

The Human TRH ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with TRH. During the reaction, Human TRH in the sample or standard competes with a fixed amount of TRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human TRH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human TRH in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Thyrotropin Releasing Hormone

Abbreviation : TRH

Uniprot ID : P20396

Format : 96 rx

Reactivity : Human

ELISA configuration : Competitive

Sensitivity : 4.688 pg/ml

Detection Range : 7.813 – 500 pg/ml

Manual & Datasheet

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