Human TPA (Tissue Polypeptide Antigen) ELISA Kit – E-EL-H2361

Human TPA (Tissue Polypeptide Antigen) ELISA Kit

The Human TPA ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TPA. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TPA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TPA. You can calculate the concentration of TPA in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Tissue Polypeptide Antigen

Abbreviation : TPA

Uniprot ID :

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

More information about this Human TPA (Tissue Polypeptide Antigen) ELISA Kit from Elabscience