Human PL (Pancreatic Lipase) ELISA Kit – E-EL-H2245

Human PL (Pancreatic Lipase) ELISA Kit

The Human PL ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PL. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for PL and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PL. You can calculate the concentration of PL in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Pancreatic Lipase

Abbreviation : PL

Uniprot ID : P16233

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 4.688 pg/ml

Detection Range : 7.813 – 500 pg/ml

Manual & Datasheet

More information about this Human PL (Pancreatic Lipase) ELISA Kit from Elabscience