Human MUC5AC (Mucin 5 Subtype AC) ELISA Kit
The Human MUC5AC ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MUC5AC. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human MUC5AC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC5AC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MUC5AC. You can calculate the concentration of Human MUC5AC in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Mucin 5 Subtype AC
Abbreviation : MUC5AC
Uniprot ID : P98088
Format : 96 rx
Reactivity : Human
ELISA configuration : Sandwich
Sensitivity : 0.188 ng/ml
Detection Range : 0.313 – 20 ng/ml
More information about this Human MUC5AC (Mucin 5 Subtype AC) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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