Human EGR1 (Early Growth Response Protein 1) ELISA Kit
The Human EGR1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to EGR1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for EGR1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain EGR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of EGR1. You can calculate the concentration of EGR1 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Early Growth Response Protein 1
Abbreviation : EGR1
Uniprot ID : P18146
Format : 96 rx
Reactivity : Human
ELISA configuration : Sandwich
Sensitivity : 0.094 ng/ml
Detection Range : 0.156 – 10 ng/ml
More information about this Human EGR1 (Early Growth Response Protein 1) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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