Human BCL2L1 / Bcl-XL Protein (His tag) – PKSH031599

Recombinant Human BCL2L1 / Bcl-XL Protein (His tag)

B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1), belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is chareacterized as important factors in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.

Synonym(s) : RP5-857M17.3 , BCL-XL/S , BCL2L , BCLX , BCLXL , BCLXS , Bcl-X , DKFZp781P2092 , bcl-xL , bcl-xS

Species(s) : Human

Protein Expression Host : E. coli

Protein Construction : A DNA sequence encoding the human BCL2L1 isoform 1 (NP_612815.1) (Met 1-Arg 212) was fused with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Met

Molecular weight/mass : The recombinant human BCL2L1 consisting of 222 amino acids and has a calculated molecular mass of 25.2 kDa. It migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human BCL2L1 isoform 1 (NP_612815.1) (Met 1-Arg 212) was fused with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile 20mM Tris, pH 8.0

Purity : > 85 % as determined by SDS-PAGE

Endotoxin level :

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet