Human BCL-W / BCL2L2 Protein (His tag) – PKSH031906

Recombinant Human BCL-W / BCL2L2 Protein (His tag)

Beta1,4-Galactosyltransferase-I (B4GALT1), one of seven beta1,4-galactosyltransferases, is an enzyme commonly found in the trans-Golgi complex that adds galactose to oligosaccharides. They have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 gene directs production of B4GALT1 protein using either of two transcription start sites. The product of the smaller transcript serves the traditional biosynthetic role in the Golgi. This form also complexes with α-lactalbumin, a mammary-specific protein, to form lactose synthase. In addition to a biosynthetic role, the protein translated from the longer transcript appears on the plasma membranes of some cells where it serves as a signalling receptor in cell-matrix interactions such as sperm-egg binding.

Synonym(s) : BCL2L2 , BCL-W , BCL2-L-2 , BCLW , KIAA0271

Species(s) : Human

Protein Expression Host : E. coli

Protein Construction : A DNA sequence encoding the human BCL-W (Q92843-1) (Met 1-Thr 172) was fused with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Met

Molecular weight/mass : The recombinant human BCL-W consisting of 182 amino acids and has a calculated molecular mass of 20 kDa. It migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human BCL-W (Q92843-1) (Met 1-Thr 172) was fused with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile 25mM Hepes 0.1MKCl 10% glycerolcerolpH 7.5

Purity : > 95 % as determined by SDS-PAGE

Endotoxin level :

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet