Human BCAM Protein (Fc tag) – PKSH031736

Recombinant Human BCAM Protein (Fc tag)

The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes.

Synonym(s) : BCAM , AU , CD239 , LU , MSK19

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the extracellular domain of human BCAM (NP_005572.2) (Met 1-Ala 547) was fused with the Fc region of human IgG1 at the C-terminus.

Predicted N-terminal amino acid : Glu 32

Molecular weight/mass : The recombinant human BCAM/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 754 amino acids and predicts a molecular mass of 83 kDa. As a result of glycosylation, the rh BCAM/Fc monomer migrates as approximately 100-110 kDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the extracellular domain of human BCAM (NP_005572.2) (Met 1-Ala 547) was fused with the Fc region of human IgG1 at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 95 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet