Human BACE1 (Beta-site APP Cleaving Enzyme 1) ELISA Kit – E-EL-H0214

Human BACE1 (Beta-site APP Cleaving Enzyme 1) ELISA Kit

The Human BACE1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BACE1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for BACE1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BACE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of BACE1. You can calculate the concentration of BACE1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Beta-site APP Cleaving Enzyme 1

Abbreviation : BACE1

Uniprot ID : P56817

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.375 ng/ml

Detection Range : 0.625 – 40 ng/ml

Manual & Datasheet

More information about this Human BACE1 (Beta-site APP Cleaving Enzyme 1) ELISA Kit from Elabscience