Human B7-H5 / Gi24 / VISTA Protein (His tag) – PKSH030625

Recombinant Human B7-H5 / Gi24 / VISTA Protein (His tag)

C10orf54, also known as GI24, belongs to the immunoglobulin superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, C10orf54 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.

Synonym(s) : C10orf54

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the human Gi24 (AAH20568.1) (Met1-Ala194) was expressed with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Phe 33

Molecular weight/mass : The recombinant human Gi24 consists of 173 amino acids and predicts a molecular mass of 19.6 KDa. It migrates as an approximately 38-42 KDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human Gi24 (AAH20568.1) (Met1-Ala194) was expressed with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 95 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet