Human B7-H4 / B7S1 / B7x Protein (His tag) – PKSH031452

Recombinant Human B7-H4 / B7S1 / B7x Protein (His tag)

V-set domain-containing T-cell activation inhibitor 1, also known as B7X, B7H4, B7S1, and VTCN1, is a single-pass type? membrane protein belonging to the B7 family of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands on T lymphocytes. They provide costimulatory signals that regulate T cell responses. A soluble form of B7H4 has also been detected. B7X / VTCN1 / B7H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7X / VTCN1 / B7H4 plays an important role, together with regulatory T-cells(Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. B7X / VTCN1 / B7H4 is also involved in promoting epithelial cell transformation. This membrane protein can be up-regulated by IL6 / interleukin-6 and IL10 / interleukin-10 and inhibited by CSF2 / GM-CSF and IL4 / interleukin-4 on antigen-presenting cells.

Synonym(s) : VTCN1 , B7H4

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the human VTCN1(Q7Z7D3) (Phe29-Ala258) with a C-terminal polyhistidine tag was expressed.

Predicted N-terminal amino acid : Phe 29

Molecular weight/mass : The recombinant human VTCN1 comprises 241 amino acids and has a predicted molecular mass of 26.8 kDa. The apparent molecular mass of the protein is approximately 43-48 kDa in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human VTCN1(Q7Z7D3) (Phe29-Ala258) with a C-terminal polyhistidine tag was expressed.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 95 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet