Human B-Cell Lymphoma 11A (Bcl11A) ELISA Kit – E-EL-H2078

Human B-Cell Lymphoma 11A (Bcl11A) ELISA Kit

The ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Bcl11A. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human Bcl11A and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Bcl11A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Bcl11A. You can calculate the concentration of Human Bcl11A in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Bcl11A

Abbreviation : B-CellLymphoma11A

Uniprot ID : Q9H165

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.375 ng/ml

Detection Range : 0.625 – 40 ng/ml

Manual & Datasheet

More information about this Human B-Cell Lymphoma 11A (Bcl11A) ELISA Kit from Elabscience