Human ATF3 (Activating Transcription Factor 3) ELISA Kit – E-EL-H0523

Human ATF3 (Activating Transcription Factor 3) ELISA Kit

The Human ATF3 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ATF-3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ATF-3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ATF-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ATF-3. You can calculate the concentration of ATF-3 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Activating Transcription Factor 3

Abbreviation : ATF3

Uniprot ID : P18847

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Human ATF3 (Activating Transcription Factor 3) ELISA Kit from Elabscience