Human APP (Amyloid Precursor Protein) ELISA Kit – E-EL-H1216

Human APP (Amyloid Precursor Protein) ELISA Kit

The Human APP ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to APP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for APP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain APP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of APP. You can calculate the concentration of APP in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Amyloid Precursor Protein

Abbreviation : APP

Uniprot ID : P05067

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

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