Human ApoE (Apolipoprotein E) ELISA Kit – E-EL-H0470

Human ApoE (Apolipoprotein E) ELISA Kit

The Human ApoE ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ApoE. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ApoE and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ApoE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ApoE. You can calculate the concentration of ApoE in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Apolipoprotein E

Abbreviation : ApoE

Uniprot ID : P02649

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 14.063 ng/ml

Detection Range : 23.438 – 1500 ng/ml

Manual & Datasheet

More information about this Human ApoE (Apolipoprotein E) ELISA Kit from Elabscience