Human Apo A1-Ab (Anti-Apolipoprotein A1 Antibody) ELISA Kit
The Human Apo A1-Ab ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Apo A1-Ab. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Apo A1-Ab and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Apo A1-Ab, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Apo A1-Ab. You can calculate the concentration of Apo A1-Ab in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Anti-Apolipoprotein A1
Abbreviation : ApoA1-Ab
Format : 96 rx
Reactivity : Human
ELISA configuration : Sandwich
Sensitivity : 1.875 ng/ml
Detection Range : 3.125 – 200 ng/ml
Additional information
Format | 96 rx |
---|
Ask a question about Human Apo A1-Ab (Anti-Apolipoprotein A1 Antibody) ELISA Kit – E-EL-H0460
You must be logged in to post a review.
Reviews