Human AP17 (Apelin 17) ELISA Kit – E-EL-H0236

Human AP17 (Apelin 17) ELISA Kit

The Human AP17 ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with AP17. During the reaction, Human AP17 in the sample or standard competes with a fixed amount of AP17 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human AP17. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human AP17 in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Apelin 17

Abbreviation : AP17

Uniprot ID :

Format : 96 rx

Reactivity : Human

ELISA configuration : Competitive

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Human AP17 (Apelin 17) ELISA Kit from Elabscience