Human ANP (Atrial Natriuretic Peptide) ELISA Kit – E-EL-H0532

Human ANP (Atrial Natriuretic Peptide) ELISA Kit

The Human ANP ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with ANP. During the reaction, Human ANP in the sample or standard competes with a fixed amount of ANP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human ANP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human ANP in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Atrial Natriuretic Peptide

Abbreviation : ANP

Uniprot ID : P01160

Format : 96 rx

Reactivity : Human

ELISA configuration : Competitive

Sensitivity : 4.688 pg/ml

Detection Range : 7.813 – 500 pg/ml

Manual & Datasheet

More information about this Human ANP (Atrial Natriuretic Peptide) ELISA Kit from Elabscience