Human ANGPTL4 (Angiopoietin Like Protein 4) ELISA Kit
The Human ANGPTL4 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ANGPTL4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ANGPTL4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ANGPTL4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ANGPTL4. You can calculate the concentration of ANGPTL4 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Angiopoietin Like Protein 4
Abbreviation : ANGPTL4
Uniprot ID : Q9BY76
Format : 96 rx
Reactivity : Human
ELISA configuration : Sandwich
Sensitivity : 0.938 ng/ml
Detection Range : 1.563 – 100 ng/ml
More information about this Human ANGPTL4 (Angiopoietin Like Protein 4) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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