Human Ang-II (Angiotensin II) ELISA Kit – E-EL-H0326

Human Ang-II (Angiotensin II) ELISA Kit

The Human Ang-II ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Human Ang-II. During the reaction, Human Ang-II in the sample or standard competes with a fixed amount of Human Ang-II on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Human Ang-II. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Ang-II in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Angiotensin II

Abbreviation : Ang-II

Uniprot ID :

Format : 96 rx

Reactivity : Human

ELISA configuration : Competitive

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

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