Human AMY2A / Alpha-amylase Protein (His tag) – PKSH030667

Recombinant Human AMY2A / Alpha-amylase Protein (His tag)

Alpha-amylase is the major form of amylase found in humans and other mammals. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. Alpha-amylase hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Amylases is widely expressed and is most prominent in pancreatic juice and saliva, each of which has its own isoform of human α-amylase. They behave differently on isoelectric focusing, and can also be separated in testing by using specific monoclonal antibodies.

Synonym(s) : AMY2A

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the human AMY2A (P04746) (Met1-Leu511) was expressed with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Gln 16

Molecular weight/mass : The recombinant human AMY2A consists of 507 amino acids and predicts a molecular mass of 57.3 KDa. It migrates as an approximately 53-58 KDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human AMY2A (P04746) (Met1-Leu511) was expressed with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 85 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet