Human AMY1 (Amylase Alpha 1, Salivary) ELISA Kit – E-EL-H0320

Human AMY1 (Amylase Alpha 1, Salivary) ELISA Kit

The Human AMY1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AMY1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AMY1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AMY1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AMY1. You can calculate the concentration of AMY1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Amylase Alpha 1, Salivary

Abbreviation : AMY1

Uniprot ID : P04745

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.938 ng/ml

Detection Range : 1.563 – 100 ng/ml

Manual & Datasheet

More information about this Human AMY1 (Amylase Alpha 1, Salivary) ELISA Kit from Elabscience