Human ALOX5AP / FLAP Protein (His tag) – PKSH031059

Recombinant Human ALOX5AP / FLAP Protein (His tag)

Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP), also known as FLAP, belongs to the MAPEG family. ALOX5AP/FLAP is an essential partner of 5-LO for this process. The FLAP (ALOX5AP) gene has been linked to risk for myocardial infarction, stroke and restenosis, reigniting pharmaceutical interest in this target. It had been found that ALOX5AP/FLAP is a key enzyme in leukotriene formation, in both human pulmonary microvascular endothelial cells and a transformed human brain endothelial cell line. In addition, the protein FLAP has recently been identified as an emerging target in metabolic disease. In fact, FLAP is overexpressed in the adipose tissue of patients and experimental animals with obesity.

Synonym(s) : FLAP , ALOX5AP

Species(s) : Human

Protein Expression Host : Baculovirus-Insect Cells

Protein Construction : A DNA sequence encoding the full length of human ALOX5AP (NP_001620.2) (Met 1-Pro 161) was fused with a polyhistidine tag at the carboxy-terminus.

Predicted N-terminal amino acid : Met 1

Molecular weight/mass : The recombinant human ALOX5AP consists of 167 amino acids and predicts a molecular mass of 19 kDa as estimated in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the full length of human ALOX5AP (NP_001620.2) (Met 1-Pro 161) was fused with a polyhistidine tag at the carboxy-terminus.

Formulation : Lyophilized from sterile 20mM Tris, 150mM NaCl, 10% glycerol, pH 7.4, 0.5% triton, 5mM DTT

Purity : > 90 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet