Human ALDH7A1 / ATQ1 Protein (His tag) – PKSH031063

Recombinant Human ALDH7A1 / ATQ1 Protein (His tag)

ALDH7A1 (Aldehyde dehydrogenase 7 family, member A1) is a member of subfamily 7 in the aldehyde dehydrogenase family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. Mammalian ALDH7A1 is homologous to plant ALDH7B1 which protects against various forms of stress such as increased salinity, dehydration and treatment with oxidants or pesticides. In mammals, ALDH7A1 is known to play a primary role during lysine catabolism through the NAD+-dependent oxidative conversion of aminoadipate semialdehyde (AASA) to its corresponding carboxylic acid, α-aminoadipic acid. Deleterious mutations in human ALDH7A1 are responsible for pyridoxine-dependent and folinic acid-responsive seizures. ALDH7A1 is a novel aldehyde dehydrogenase expressed in multiple subcellular compartments that protects against hyperosmotic stress by generating osmolytes and metabolizing toxic aldehydes.

Synonym(s) : ATQ1 , EPD , FLJ11738 , FLJ92814 , PDE , ALDH7A1

Species(s) : Human

Protein Expression Host : E. coli

Protein Construction : A DNA sequence encoding the human ALDH7A1 isoform 2 (P49419-2) (Ser 2-Gln 511) was expressed, with a polyhistide tag at the N-terminus.

Predicted N-terminal amino acid : Met

Molecular weight/mass : The recombinant human ALDH7A1 consisting of 517 amino acids and migrates as an approximately 56 kDa band in SDS-PAGE under reducing conditions as predicted.

Protein Construction : A DNA sequence encoding the human ALDH7A1 isoform 2 (P49419-2) (Ser 2-Gln 511) was expressed, with a polyhistide tag at the N-terminus.

Formulation : Lyophilized from sterile 50mM Tris, 500mM NaCl, 20% glycerol, pH 8.0

Purity : > 93 % as determined by SDS-PAGE

Endotoxin level :

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet