Human AKR1B1 Protein (His tag) – PKSH031165

Recombinant Human AKR1B1 Protein (His tag)

Aldose reductase (AKR1B1) belongs to the aldo/keto reductase superfamily. AKR1B1 is a NADPH-dependent aldo-keto reductase best known as the rate-limiting enzyme of the polyol pathway. Expression of AKR1B1 was the highest in lens and retina. It is the first enzyme in the polyol pathway through which glucose is converted to sorbitol which is important for the function of various organs in the body, and has been implicated in the etiology of diabetic complications. AKR1B1 is quite abundant in the collecting tubule cells and thought to provide protection against hypertonic environment. Some human tissues contain AKR1B1 as well as AKR1B10, a closely related member of the aldo-keto reductase superfamily.

Synonym(s) : ADR , ALDR1 , ALR2 , AR , MGC1804

Species(s) : Human

Protein Expression Host : E. coli

Protein Construction : A DNA sequence encoding the human AKR1B1 (P15121) (Met 1-Phe 316) was expressed, with a polyhistidine tag at the N-terminus.

Predicted N-terminal amino acid : Met

Molecular weight/mass : The recombinant human AKR1B1 comprises 332 amino acids and has a predicted molecular mass of 37.9 kDa. It migrates as an approximately 36 kDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human AKR1B1 (P15121) (Met 1-Phe 316) was expressed, with a polyhistidine tag at the N-terminus.

Formulation : Lyophilized from sterile PBS, 20% glycerol, pH 7.5

Purity : > 90 % as determined by SDS-PAGE

Endotoxin level :

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet