Human AKR1A1 Protein (His tag) – PKSH031070

Recombinant Human AKR1A1 Protein (His tag)

Aldehyde reductase (AKR1A1) is a member of the aldo-keto reductase superfamily, which consists of more than 40 known enzymes and proteins that includes variety of monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase. Aldehyde reductase has wide substrate specificities for carbonyl compounds. These enzymes are implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Aldehyde reductase possess a structure with a beta-alpha-beta fold which contains a novel NADP-binding motif. The binding site is located in a large, deep, elliptical pocket in the C-terminal end of the beta sheet, the substrate being bound in an extended conformation. This binding is more similar to FAD- than to NAD(P)-binding oxidoreductases. AKR1A1 is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue.

Synonym(s) : RP4-697E16.2 , ALDR1 , ALR , ARM , DD3 , MGC12529 , MGC1380

Species(s) : Human

Protein Expression Host : E. coli

Protein Construction : A DNA sequence encoding the human AKR1A1 (P14550) (Met 1-Tyr 325) was expressed, with a polyhistidine tag at the N-terminus.

Predicted N-terminal amino acid : Met

Molecular weight/mass : The recombinant human AKR1A1 consisting of 341 amino acids and migrates as an approximately 39 kDa band in SDS-PAGE under reducing conditions as predicted.

Protein Construction : A DNA sequence encoding the human AKR1A1 (P14550) (Met 1-Tyr 325) was expressed, with a polyhistidine tag at the N-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.5

Purity : > 90 % as determined by SDS-PAGE

Endotoxin level :

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet