Human ADK Protein (His & GST tag) – PKSH030331

Recombinant Human ADK Protein (His & GST tag)

Adenosine kinase(ADK) belongs to the family of transferases. Adenosine kinase (ADK) is the key enzyme in adenosine metabolism and catalyzes ATP and adenosine into two products: ADP and AMP. Two isoforms of the enzyme adenosine kinase (ADK), which differ at their N-terminal ends, are found in mammalian cells. It has been shown that the two ADK isoforms differ only in their first exons and the promoter regions; hence they arise via differential splicing of their first exons with the other exons common to both isoforms. In adult brain, ADK is primarily present in astrocytes. Several lines of experimental evidence support a critical role of ADK in different types of brain injury associated with astrogliosis, which is also a prominent morphologic feature of temporal lobe epilepsy (TLE). It has been suggested that dysregulation of ADK in astrocytes is a common pathologic hallmark of TLE. Moreover, in vitro data suggest the existence of an additional layer of modulatory crosstalk between the astrocyte-based adenosine cycle and inflammation. ADK also contributes to CK homeostasis in vivo.

Synonym(s) : AK

Species(s) : Human

Protein Expression Host : Baculovirus-Insect Cells

Protein Construction : A DNA sequence encoding the human ADK isoform short (AAH03568.1) (Met 1-His 345) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Predicted N-terminal amino acid : Met

Molecular weight/mass : The recombinant human ADK/GST chimera consists of 582 amino acids and has a calculated molecular mass of 68 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human ADK isoform short (AAH03568.1) (Met 1-His 345) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Formulation : Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol, 0.3mM DTT

Purity : > 90 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet