Human ADAMTSL1 / PUNCTIN Protein (His tag) – PKSH030600

Recombinant Human ADAMTSL1 / PUNCTIN Protein (His tag)

ADAMTSL1 is a secreted molecule resembling members of the ADAMTS protein family of matrix metalloproteinases. Both ADAMTS proteins and ADAM protein family contain a disintegrin and a metalloprotease domain. Metallospondins is collective term for members of ADAMTS protein family. ADAMTS proteins lack the EGF-like domain found normally in members of the ADAM protein family. They also do not possess the canonical disintegrin sequence found in the ADAM protein family. It contains the domains found in members of the ADAMTS protein family with the exception of the pro-metalloprotease and the disintegrin-like domain typical of this family. ADAMTSL1 gene is expressed in adult skeletal muscle. ADAMTSL1 may play an important role in the extracellular matrix as it is deposited in the cell substratum in a punctate fashion and is excluded from focal contacts.

Synonym(s) : ADAMTSL1 , ADAMTSR1 , C9orf94

Species(s) : Human

Protein Expression Host : Baculovirus-Insect Cells

Protein Construction : A DNA sequence encoding the human ADAMTSL1 (Met 1-His439) (Q8N6G6-2) was expressed, with a C-terminal polyhistidine tag.

Predicted N-terminal amino acid : Ile 24

Molecular weight/mass : The secreted recombinant human ADAMTSL1 consists of 421 amino acids and predicts a molecular mass of 47 KDa. The apparent molecular mass of the protein is approximately 48 Kda in SDS-PAGE under reducing conditions due to glycosylation.

Protein Construction : A DNA sequence encoding the human ADAMTSL1 (Met 1-His439) (Q8N6G6-2) was expressed, with a C-terminal polyhistidine tag.

Formulation : Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4

Purity : > 97 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet