Human ACP5 / TRAP Protein (His tag) – PKSH031534

Recombinant Human ACP5 / TRAP Protein (His tag)

Tartrate-resistant acid phosphatase (TRACP) or acid phosphatase 5, tartrate resistant (ACP5 or TRAP) is a glycosylated monomeric metalloenzyme expressed in mammals. TRACP is associated with osteoblast migration to bone resorption sites, and, once there, TRACP is believed to initiate osteoblast differentiation, activation, and proliferation. TRACP once considered to be just a histochemical marker of osteoclasts is now recognised to be a molecule of widespread occurrence with functions in both the skeleton and the immune system. Two forms of TRACP circulate in human blood, TRACP 5a derived from macrophages and dendritic cells, and TRACP-5b derived from osteoclasts. Recent data have demonstrated the utility of TRACP-5b as a marker of osteoclast number and bone resorption, and serum TRACP-5a as a marker of inflammatory conditions. TRACP is expressed by osteoclasts, macrophages, dendritic cells and a number of other cell types. It has a critical role in many biological processes including skeletal development, collagen synthesis and degradation, the mineralisation of bone, cytokine production by macrophages and dendritic cells, macrophage recruitment, dendritic cell maturation and a role in the development of Th1 responses.

Synonym(s) : ACP5 , MGC117378 , TRAP

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the human ACP5 (NP_001602.1) precursor (Met 1-Pro 320) was expressed with a C-terminal polyhistidine tag.

Predicted N-terminal amino acid : Ala 22

Molecular weight/mass : The secreted recombinant human ACP5 comprises 310 amino acids with a predicted molecular mass of 35 kDa as estimated by SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human ACP5 (NP_001602.1) precursor (Met 1-Pro 320) was expressed with a C-terminal polyhistidine tag.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 95 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet