Human ACAT2 (Acetyl Coenzyme A Acetyltransferase 2, cytosolic) ELISA Kit - E-EL-H0139

Human ACAT2 (Acetyl Coenzyme A Acetyltransferase 2, cytosolic) ELISA Kit

The Human ACAT2 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ACAT2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ACAT2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ACAT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ACAT2. You can calculate the concentration of ACAT2 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Acetyl Coenzyme A Acetyltransferase 2, cytosolic

Abbreviation : ACAT2

Uniprot ID : Q9BWD1

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 - 5000 pg/ml

Manual & Datasheet

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