Chicken SCF (Stem Cell Factor) ELISA Kit – E-EL-Ch1585

Chicken SCF (Stem Cell Factor) ELISA Kit

The Chicken SCF ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SCF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for SCF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SCF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of SCF. You can calculate the concentration of SCF in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Chicken Stem Cell Factor

Abbreviation : SCF

Uniprot ID : Q09108

Format : 96 rx

Reactivity : Chicken

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

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