Chicken PTH (Parathyroid Hormone) ELISA Kit – E-EL-Ch0433

Chicken PTH (Parathyroid Hormone) ELISA Kit

The Chicken PTH ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Chicken PTH. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Chicken PTH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Chicken PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Chicken PTH. You can calculate the concentration of Chicken PTH in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Chicken Parathyroid Hormone

Abbreviation : PTH

Uniprot ID : P15743

Format : 96 rx

Reactivity : Chicken

ELISA configuration : Sandwich

Sensitivity : 75 pg/ml

Detection Range : 125 – 8000 pg/ml

Manual & Datasheet

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