Chicken IgY (Immunoglobulin Y) ELISA Kit – E-EL-Ch0001

Chicken IgY (Immunoglobulin Y) ELISA Kit

The Chicken IgY ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IgY. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for IgY and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IgY, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of IgY. You can calculate the concentration of IgY in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Chicken Immunoglobulin Y

Abbreviation : IgY

Uniprot ID :

Format : 96 rx

Reactivity : Chicken

ELISA configuration : Sandwich

Sensitivity : 0.469 ng/ml

Detection Range : 0.781 – 50 ng/ml

Manual & Datasheet

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