Chicken IgM (Immunoglobulin M) ELISA Kit – E-EL-Ch0367

Chicken IgM (Immunoglobulin M) ELISA Kit

The Chicken IgM ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Chicken IgM. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Chicken IgM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Chicken IgM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Chicken IgM. You can calculate the concentration of Chicken IgM in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Chicken Immunoglobulin M

Abbreviation : IgM

Uniprot ID :

Format : 96 rx

Reactivity : Chicken

ELISA configuration : Sandwich

Sensitivity : 4.688 ng/ml

Detection Range : 7.813 – 500 ng/ml

Manual & Datasheet

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