Chicken IGF-1 (Insulin Like Growth Factor 1) ELISA Kit – E-EL-Ch0116

Chicken IGF-1 (Insulin Like Growth Factor 1) ELISA Kit

The Chicken IGF-1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IGF-1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of IGF-1. You can calculate the concentration of IGF-1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Chicken Insulin Like Growth Factor 1

Abbreviation : IGF-1

Uniprot ID : P18254

Format : 96 rx

Reactivity : Chicken

ELISA configuration : Sandwich

Sensitivity : 11.25 pg/ml

Detection Range : 18.75 – 1200 pg/ml

Manual & Datasheet

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