FastPfu FLY (Ultra-HiFi) DNA PolymeraseSetting Higher Standards of High-Fidelity DNA Polymerase Performance PCR with FLY Today !
Features of FastPfu FLY
Low or High GC Content
FLY is Starred in our PCR Success Stories
FastPfu FLY helped solve > 10 customer problems in the past year
PCR Success Story #9: High-Fidelity Amplification from Human gDNA
Perfect amplification of a segment of human SSPO from gDNA with FastPfu FLY.
PCR Success Story #14: Cloning of murC and murE from Pseudomonas aeruginosa gDNA
High-GC Content PCR
PCR Amplification of murE using FastPfu and FastPfu FLY.
The murE gene has an overall GC content of 69% GC with some segments reaching 80% GC.
Success Story # 20: Vector Amplification by Fast & Steep PCR
Low GC content and Long Range PCR
Successful Vector Amplification using FLY and TransBionovo DNA Polymerases.
Phusion and Q5 failed.
Detection Limit : 25 pg gDNA
Ranking of High-Fidelity DNA Polymerases:
- Detection limit: FLY > Q5 = KKP > FastPfu > KP > Phusion
- Yield: FLY > Q5 ≥ KKP > KP ≥ FastPfu ≥ Phusion
GC-rich PCR from gDNAPCR amplification of human ARX 658 bp (79% GC) using a gradient of PCR Stimulant.
FastPfu FLY did not produce non-specific bands.
Permissive PCR Cycling Conditions
FastPfu FLY requires very little PCR Cycling Optimization.
Wide window of perfect annealing temperature, even on purified or un-purified gDNA.
FLY your PCRs
FLY is faster than any DNA Polymerase tested on the market.
Its extension time reaches 6 kb/min on simple templates (plasmid and cDNA) and often on gDNA templates.