Rat Cathepsin B / CTSB Protein (His Tag) – PKSR030155

Recombinant Rat Cathepsin B / CTSB Protein (His Tag)

Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.

Synonym(s) : Cathepsin B , CTSB

Species(s) : Rat

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the rat Ctsb (NP_072119.2) (Met1-Phe339) was expressed with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : His 18

Molecular weight/mass : The recombinant rat Ctsb consists 333 amino acids and predicts a molecular mass of 37.1 kDa.

Protein Construction : A DNA sequence encoding the rat Ctsb (NP_072119.2) (Met1-Phe339) was expressed with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile 25 mM Tris, 150 mM NaCl, pH 7.5

Purity : > 95 % as determined by SDS-PAGE.

Endotoxin level : < 1.0 EU per μg protein as determined by the LAL method.

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet