Select Page

MagicPure RNA Purification Magnetic Beads – EC501

44.00$3,232.00$ CAD

Clear

Cashback Reward : Earn up to 323$!


MagicPure RNA Purification Magnetic Beads

MagicPure™ RNA Purification Magnetic beads can easily extract RNA from mastermix of reaction such as rRNA depletion, DNase I digestion, in vitro transcription, labeled RNA and synthetic RNA. The resulting RNA product is suitable for building RNA libraries, RT-PCR, qRT-PCR, chip analysis, Northern blot RNAi, or other downstream applications.


Components provided with MagicPure RNA Purification Magnetic Beads

RNA Purification Magnetic Beads / RNase-Free Water
EC401-01: 1 ml / 1 ml
EC401-02: 5 ml / 5 ml
EC401-03: 60 ml / 60 ml


Procedure for RNA Purification using MagicPure RNABeads

Reagents to be supplied by users: freshly prepared 80% ethanol (made with RNase-free Water)
Use at room temperature.

1.8× beads are recommended for the purification.

  1. Take out the beads from 4°C refrigerator and equilibrate at room temperature for 30 minutes before use.
  2. Add RNA sample into a 1.5 ml RNase-free tube.
  3. Re-suspend RNA beads by vortexing. Add appropriate volume of beads to the sample according to the sample volume.
    Volume of beads to add = volume of sample x 1.8
    Example: 90 μl (beads to add) = 50 μl x 1.8
  4. Mix by pipetting up and down. Incubate at room temperature for 5 minutes.
    Note: Insufficient mixing will affect the results significantly.
  5. Place the tube on an appropriate magnetic stand to separate beads from the supernatant at room temperature. When the solution is
    clear (about 5 minutes), discard the supernatant.
    Note: Spin down briefly before put on magnetic stand if there is liquid on the wall. Make sure that RNA beads are settled to the
    magnet completely and not to be disturbed when discarding the supernatant. Discarding beads will result in reduced yield.
  6. With the tube still on the magnetic stand, add 200 μl of freshly prepared 80% ethanol (made with RNase-free Water) and incubate at
    room temperature for 30 seconds without pipetting up and down. Carefully remove and discard the supernatant.
    Note: Use freshly-prepared 80% ethanol; otherwise it may affect the result.
  7. Repeat step 6 one time.
  8. Air dry the beads for up to 5 minutes while the tube is on the magnetic stand with the lid open.
    Note: Residual ethanol may influence the downstream reaction. Do not over dry or heat the beads, which may result in reduced yield.
  9. Remove the tube from the magnetic stand and elute RNA with ≥ 20 μl of RNase-free Water. Mix by pipetting up and down or
    vortexing. Then incubate at room temperature for 2 minutes.
  10. Put the tube back to the magnetic stand. Incubate for 2 minutes (or until the solution is clear).
    Note: Spin down briefly before put on magnetic stand if there is liquid on the wall. Prolong incubation to 5 minutes if necessary to
    make sure that RNA beads are settled to the magnet completely.
  11. Transfer the supernatant to a new RNase-free tube. The RNA product can be stored at -80°C.

qPCR data from RNA purified samples

EC501 MagicPure RNA beads qPCR data1


Manual & Datasheet

EC501 MagicPure RNA Beads

Additional information

Format

1 ml, 5 ml, 60 ml, 450 ml

Supplier

Ask a question about MagicPure RNA Purification Magnetic Beads – EC501

Product Reviews for MagicPure RNA Purification Magnetic Beads – EC501

Reviews

There are no reviews yet.

Be the first to review “MagicPure RNA Purification Magnetic Beads – EC501”

Your email address will not be published. Required fields are marked *

Related Products

More to Read

PCR Purification Kit Comparison – DNA Clean Up

PCR Purification Kit Comparison – DNA Clean Up

Which is the Best Kit for DNA Clean Up? Our aim was to perform a PCR Purification Kit Comparison from different vendors. At Civic Bioscience, we distribute the Favorprep™ and EasyPure® product lines from FAVORGEN and TransBionovo respectively. First, the FavorPrep™...

The Truth about Antibodies used for Research

The Truth about Antibodies used for Research

The Problem with Antibodies used for Research Antibodies used for Research in academic, industrial and therapeutic fields are universal reagents in molecular biology assays. Without them, the entire field of medical research could collapse. However, there are many...

Detection Limit of Taq DNA Polymerase

Detection Limit of Taq DNA Polymerase

What's the Detection Limit of Taq ? How many DNA copies from gDNA? There are MANY Taq DNA Polymerases out there on the market. Some are driven by strong marketing and others by strong performance. Our aim was to verify the Detection Limit of Taq DNA Polymerases in PCR...

4th Anniversary Celebration | 2019-2020 Price List Available

by Simon Roy | Sep 16th, 2019 | Latest News, Promotions |This offer is not available yet. Please check again tomorrow.  20% discount on selected cell biology products: TransDetect® Cell Counting Kit (CCK)-FC101 TransDetect® Annexin V-FITC/PI Cell Apoptosis...

The 2019-2020 Price List is now available

A huge THANK YOU for your support! MERCI encore INFINIMENT de supporter notre jeune compagnie!   by Simon Roy | September 16th, 2019 | Company Posts, Latest NewsPrices for services vary case by case. 4rth anniversary promotions are coming soon! Let's CELEBRATE...

Limited Offer – Get a Free mini16 Thermocycler!

Limited Offer – Get a Free mini16 Thermocycler!

For my own birthday, every year, I find a way to celebrate with you. To be quite frank, the promotion I want to do is, for the moment, not configurable on the website but I will work on it in the coming days so that it becomes possible by Valentine’s Day. However, I...