Rat TNF-α (Tumor Necrosis Factor Alpha) CLIA Kit – E-CL-R0019

Rat TNF-α (Tumor Necrosis Factor Alpha) CLIA Kit

The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TNF-α. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the;specific antibody. Then a biotinylated detection antibody specific for Rat TNF-α and Avidin-Horseradish;Peroxidase (HRP) conjugate is added to each micro plate well successively and;incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNF-α, biotinylated detection;antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence;immunoassay analyzer. The RLU value is positively associated with the;concentration of Rat TNF-α. You can calculate the;concentration of Rat TNF-α in the samples by;comparing the RLU value of the samples to the standard curve.

Detected Target: Rat TNF-α

Alternate names: DIF, TNF-alpha, TNFA, TNFSF2

Format : 96 rx

Reactivity : Rat TNF-α

Assay configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 to 1000 pg/ml

Manual & Datasheet

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