Rat Tie2 / TEK Protein (Fc tag) – PKSR030377

Recombinant Rat Tie2 / TEK Protein (Fc tag)

TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Synonym(s) : Tie-2 , Tie2

Species(s) : Rat

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the rat TEK (NP_001099207.1) extracellular domain (Met 4-Leu 743) was fused with the Fc region of human IgG1 at the C-terminus.

Predicted N-terminal amino acid : Ala 23

Molecular weight/mass : The recombinant rat TEK/Fc is a disulfide-linked homodimer. The reduced monomer comprises 962 amino acids and predicts a molecular mass of 108 kDa. The apparent molecular mass of the rat TEK/Fc monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Protein Construction : A DNA sequence encoding the rat TEK (NP_001099207.1) extracellular domain (Met 4-Leu 743) was fused with the Fc region of human IgG1 at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 95 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet