Rat KIM-1 / TIM1 / HACVR1 Protein (His & Fc tag) – PKSR030412

Recombinant Rat KIM-1 / TIM1 / HACVR1 Protein (His & Fc tag)

Human HAV cellular receptor 1 (HAVCR1), also known as Kidney injury molecule 1 (KIM-1) and T cell immunoglobulinmucin 1 (TIM-1), is a type â… integral membrane glycoprotein. KIM-1 protein is widely expressed with highest levels in kidney and testis. It has been shown to play a major role as a human susceptibility gene for asthma, allergy and autoimmunity. IgA1lambda is a specific ligand of KIM-1 protein and that their association has a synergistic effect in virus-receptor interactions. KIM-1 involves in the pathogenesis of acute kidney injury. It had been confirmed that KIM-1 is a human urinary renal dysfunction biomarker. Moreover, KIM-1 protein is a novel regulatory molecule of flow-induced calcium signaling.

Synonym(s) : KIM-1 , Kim1 , Havcr1

Species(s) : Rat

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the extracellular domain of rat HAVCR1 (NP_775172.1) (Ser 18-Val 238) was fused with a polyhistidine tag at the C-terminus and the Fc region of human IgG1 at the N-terminus.

Predicted N-terminal amino acid : Glu 20

Molecular weight/mass : The recombinant rat HAVCR1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 468 amino acids and predicts a molecular mass of 52.6 kDa. As a result of glycosylation, the apparent molecular mass of the rat HAVCR1/Fc monomer is approximately 90-100 kDa in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the extracellular domain of rat HAVCR1 (NP_775172.1) (Ser 18-Val 238) was fused with a polyhistidine tag at the C-terminus and the Fc region of human IgG1 at the N-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 93 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet