Recombinant Rat IL1R1 / CD121a Protein (His & Fc tag)
Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.
Synonym(s) : Il1r1
Species(s) : Rat
Protein Expression Host : Human Cells
Protein Construction : A DNA sequence encoding the rat IL1R1 (NP_037255.3) extracellular domain (Met 1-Lys 352) was was fused with the C-terminal His-tagged Fc region of mouse IgG1 at the C-terminus.
Predicted N-terminal amino acid : Leu 34
Molecular weight/mass : The recombinant rat IL1R1/mFc is a disulfide-linked homodimer. The reduced monomer comprises 562 amino acids and predicts a molecular mass of 64.6 kDa. The apparent molecular mass of the ratIL1R1/mFc monomer is approximately 90-95 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Protein Construction : A DNA sequence encoding the rat IL1R1 (NP_037255.3) extracellular domain (Met 1-Lys 352) was was fused with the C-terminal His-tagged Fc region of mouse IgG1 at the C-terminus.
Formulation : Lyophilized from sterile PBS, pH 7.4
Purity : > 85 % as determined by SDS-PAGE
Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method
Stability : Samples are stable for up to twelve months from date of receipt at -70°C
Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Reconstitution : Please refer to the printed manual for detailed information.
Additional information
Format | 100 ug, 50 ug |
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Species | Rat |
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