Rat HER3 / ErbB3 Protein (His tag) – PKSR030358

Recombinant Rat HER3 / ErbB3 Protein (His tag)

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

Synonym(s) : ERBB3

Species(s) : Rat

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the rat ERBB3 (Q3MHC0) (Met1-His641) was expressed, fused with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Ser 20

Molecular weight/mass : The recombinant rat ERBB3 comprises 633 amino acids and predicts a molecular mass of 69.9 kDa. The apparent molecular mass of the recombinant protein is approximately 112 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Protein Construction : A DNA sequence encoding the rat ERBB3 (Q3MHC0) (Met1-His641) was expressed, fused with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 95 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet